Reporting on-line today in the Journal of Neurosurgery: Pediatrics.

3D-printed models of children’s brain anatomy lessen operative risk of complex procedures Boston Children's Hospital physicians report the first cases of children profiting from 3D printing of their anatomy before undergoing high-risk brain techniques. The four kids had life-threatening cerebrovascular malformations that posed particular treatment challenges . Reporting on-line today in the Journal of Neurosurgery: Pediatrics, the physicians describe the use of 3D synthetic and printing resins to create custom, high-fidelity types of the children's vessel malformations along with close by normal arteries.

Tumor spheroid expansion is certainly visualized microscopically and can be quantitated through image analysis software program for real-time and label free of charge evaluation. At the conclusion of the assay, cell viability might be assessed by fluorescence using Resazurin. The 3D Spheroid Fluorometric Proliferation/Viability Assay provides an in vitro, standardized, three-dimensional, high content format for inducing multicellular tumor spheroid formation and quantitating cell viability within the spheroids in response to pharmacological treatment. The AMSBIO 3D Spheroid Proliferation/Viability Assay establishes physiological gradients for nutrients, oxygen, catabolites, and pH due to restrictions in diffusion through multicellular layers.